胡凱鋒
一、個人簡介
胡凱鋒，博士、教授、博士生導師、畢業(yè)于瑞士聯(lián)邦蘇黎世高等理工學院，獲液態(tài)核磁共振技術博士，曾在美國NIH任研究助理，美國威斯康星大學任助理科學家?，F(xiàn)任成都中醫(yī)藥大學教授，課題組長，四川省“特聘專家”；兼任Phytochemical Analysis (PCA) 編委，中國生物物理學會代謝組學分會理事，中國生物物理學會生物磁共振專業(yè)委員會委員。
主要結(jié)合核磁共振技術和液質(zhì)聯(lián)用技術開展以下領域研究：（1）代謝組學；（2）結(jié)構(gòu)生物學；（3）復雜混合物核磁共振和液質(zhì)聯(lián)用技術定性、定量方法開發(fā);（4）中藥藥效物質(zhì)基礎與作用機制。
注重理論和技術方法創(chuàng)新，開發(fā)了一序列用于生物大分子研究的核磁共振新方法，以及用于代謝組學研究的定性、定量分析方法。拓展新技術、新方法的應用研究，借鑒代謝組學分析策略用于藥效物質(zhì)成分分析，開展疾病相關蛋白質(zhì)的結(jié)構(gòu)與功能關系研究，發(fā)展改良核磁共振方法用于靶向蛋白質(zhì)的活性分子篩選研究。發(fā)表SCI論文近50多篇。
研究組網(wǎng)http://www.hunmrlab.cn/
二、代表性成果
1.Huan Du, Xiu Gu, Jialuo Chen, Caihong Bai, Xiaohui Duan, Kaifeng Hu*, GIPMA: Global intensity-guided peak matching and alignment for 2D 1H-13C HSQC based metabolomics, Analytical Chemistry, 2023, 95(6):3195-3203
2.Caihong Bai, Suyun Xu, Jingyi Tang, Yuxi Zhang, Jiahui Yang*, Kaifeng Hu*, A ‘shape-orientated’ algorithm employing an adapted Marr wavelet and shape matching index improves the performance of continuous wavelet transform for chromatographic peak detection and quantification, Journal of Chromatography A, 2022, 1673, 463086
3.Xiu Gu, Shu Zhu*, Huan Du, Caihong Bai, Xiaohui Duan, Yiming Li, Kaifeng Hu*, Comprehensive multi-component analysis for authentication and differentiation of 6 Dendrobium species by 2D NMR-based metabolomic profiling, Microchemical Journal, 2022, 176, 107225
4.Tao Huang; Pengyu Chen; Bin Liu; Xing Li; Xinqiao Lv; Kaifeng Hu*; NPid: an automatic approach to rapid identification of known natural products in the crude extract of crabapple based on 2D 1H-13C heteronuclear correlation spectra of the extract mixture, Analytical Chemistry, 2020, 92(16), 10996-11006.
5.Xing Li; Huan Luo; Tao Huang; Li Xu; Xiaohuo Shi; Kaifeng Hu*; Statistically correlating NMR spectra and LC-MS data to facilitate the identification of individual metabolite in metabolomics mixtures, Analytical and Bioanalytical Chemistry, 2019, 411(7), 1301-1309
6.Tao Huang; Lingling Yu; Xiaofang Ma; Kaifeng Hu*; An adapted isotope dilution 1H-13C heteronuclear single-quantum correlation (ID-HSQC) for rapid and accurate quantification of endogenous and exogenous plasma glucose, Analytical and Bioanalytical Chemistry, 2018, 410(26), 6705-6711.
7.Lingling Yu; Chao Wen; Xing Li; Shiqi Fang; Lichuan Yang; Tony Wang; Kaifeng Hu*; Simultaneous quantification of endogenous and exogenous plasma glucose by isotope dilution LC-MS/MS with indirect MRM of the derivative tag, Analytical and Bioanalytical Chemistry, 2018, 410(7), 2011-2018.
8.Chao Wen; Dongshan Wang; Xing Li; Tao Huang; Cheng Huang; Kaifeng Hu*; Targeted isolation and identification of bioactive compounds lowering cholesterol in the crude extracts of crabapples using UPLC-DAD-MS-SPE/NMR based on pharmacology-guided PLS-DA, Journal of Pharmaceutical and Biomedical Analysis, 2018, 150，144-151.
9.Xing Li; Kaifeng Hu*; Quantitative NMR studies of multiple compound mixtures, in: G.A. Webb (Eds.), Annual Reports on NMR Spectroscopy, Academic Press, Elsevier, Oxford, 2017, 90, 85-143. (Invited book chapter)
10.Kaifeng Hu, William M. Westler and John L. Markley*, Two-dimensional concurrent HMQC-COSY as an approach for small molecule chemical shift assignment and compound identification. J. Bio. NMR. 2011, 49(3-4), 291-6.
11.Roger A. Chylla*, Kaifeng Hu, James J. Ellinger and John L. Markley, Deconvolution of Two-Dimensional NMR by Fast Maximum Likelihood Reconstruction (FMLR): Application to Quantitative Metabolomics. Analytical Chemistry. 2011, 83(12), 4871-80.
12.Kaifeng Hu, Thomas P. Wyche, Tim S. Bugni and John L. Markley*, Selective Quantification by 2D HSQC0 Spectroscopy of Thiocoraline in an Extract from a Sponge-Derived Verrucosispora sp. Journal of Natural Products, 2011, 74(10), 2295-8.
13.Kaifeng Hu*, James J. Ellinger, Roger A. Chylla and John L. Markley, Measurement of absolute concentrations of individual compounds in metabolite mixtures by gradient-selective time-zero 1H-13C HSQC (gsHSQC0) with two concentration references and fast maximum likelihood reconstruction analysis. Analytical Chemistry, 2011, 83(24), 9352-60.
14.Kaifeng Hu, William M. Westler and John L. Markley*, Simultaneous quantification and identification of individual chemicals in metabolite mixtures by two-dimensional extrapolated time-zero 1H-13C HSQC (HSQC0). J. Am. Chem. Soc., 2011, 133(6), 1662-5.